Solution and solid-phase peptide synthesis through Manaual and Automated peptide synthesizer (Syro-I)

Purification by HPLC (Waters, Agilent) and characterization by HRMS, MALDI-TOF (Bruker).

Conformational analysis by 1D-NMR, 2D-NMR (Bruker-400, Jeol-500, Jeol-600) and CD-spectroscopy (JASCO-1500)

Single-crystal X-ray diffraction (Bruker), and structure solution and visualization using APEX, Olex, and Mercury.

Protease stability studies to evaluate the enzymatic stability of bioactive peptides using proteolytic degradation assays.

Cell culture studies to evaluate the biological activity, cellular compatibility, and cytotoxicity of bioactive peptides.

Computational Chemistry- Ab initio and DFT calculations, NBO analysis, AIM analysis, MD simulation.